5BPQ
Crystal structure of the cysteine-rich domain of human Frizzled 4 - Crystal Form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 61 |
Unit cell lengths | 76.075, 76.075, 204.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.880 - 2.400 |
R-factor | 0.2058 |
Rwork | 0.204 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bpb |
RMSD bond length | 0.005 |
RMSD bond angle | 1.075 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.370 | 47.370 | 2.490 |
High resolution limit [Å] | 2.400 | 8.980 | 2.400 |
Rmerge | 0.046 | 0.022 | 0.606 |
Rpim | 0.026 | 0.013 | 0.339 |
Total number of observations | 103121 | 1773 | 11157 |
Number of reflections | 25975 | ||
<I/σ(I)> | 14.5 | 37.7 | 2.2 |
Completeness [%] | 99.5 | 95.3 | 99.4 |
Redundancy | 4 | 3.6 | 4.1 |
CC(1/2) | 0.999 | 0.999 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M HEPES, pH 7.5, 0.1 M NaCl, 1.6 M ammonium sulfate |