5B74
Crystal structure of conjoined Pyrococcus furiosus L-asparaginase with peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-05-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 92.010, 92.010, 187.081 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.109 - 2.039 |
R-factor | 0.2142 |
Rwork | 0.213 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ra9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.041 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.110 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.110 | 0.640 |
Number of reflections | 29314 | |
<I/σ(I)> | 13.2 | 3.4 |
Completeness [%] | 94.5 | 82 |
Redundancy | 13.5 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Sodium citrate tribasic dihydrate, 0.1 M Sodium cacodylate 6, 30%(v/v) 2-Propanol |