5B71
Crystal structure of complement C5 in complex with SKY59
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-02-05 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 39.790, 55.100, 127.760 |
Unit cell angles | 89.18, 86.24, 78.20 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.490 | 2.160 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.072 | 0.317 |
Number of reflections | 56154 | |
<I/σ(I)> | 7.9 | 1.8 |
Completeness [%] | 92.1 | 95.8 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M magnesium formate dehydrate, 15.0% w/v polyethylene glycol 3350, 20% v/v glycerol as cryoprotectant |