5B48
2-Oxoacid:Ferredoxin Oxidoreductase 1 from Sulfolobus tokodai
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-24 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.9780 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.150, 145.878, 170.094 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.470 - 2.500 |
R-factor | 0.21466 |
Rwork | 0.211 |
R-free | 0.27799 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b46 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.762 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.058 | 0.373 |
Number of reflections | 65171 | |
<I/σ(I)> | 27 | 1.72 |
Completeness [%] | 99.2 | 93.7 |
Redundancy | 4.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 15% PEG3350, 0.1 M Tris-HCl, 0.1 M NaBr |