5B2L
A crucial role of Cys218 in the stabilization of an unprecedented auto-inhibition form of MAP2K7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-26 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.985, 65.445, 88.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.940 - 2.100 |
R-factor | 0.19579 |
Rwork | 0.192 |
R-free | 0.26352 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dyl |
RMSD bond length | 0.019 |
RMSD bond angle | 1.992 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 19940 |
<I/σ(I)> | 47.6 |
Completeness [%] | 100.0 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 310 | PEG 3350, sodium citrate tribasic, HEPES |