5B1H
Crystal structure of cystathionine beta-synthase from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.890, 146.340, 82.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.490 - 2.400 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3d |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | AMoRE |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.064 | 0.412 |
| Number of reflections | 62935 | |
| <I/σ(I)> | 19.8 | 2 |
| Completeness [%] | 97.6 | 85 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | lithium sulfate, hepes |
