5AIR
Structural analysis of mouse GSK3beta fused with LRP6 peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-23 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.902, 85.883, 177.471 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.530 |
R-factor | 0.21451 |
Rwork | 0.212 |
R-free | 0.25983 |
Structure solution method | OTHER |
RMSD bond length | 0.014 |
RMSD bond angle | 1.726 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 177.500 |
High resolution limit [Å] | 2.530 |
Rmerge | 0.140 |
Number of reflections | 42941 |
<I/σ(I)> | 15.37 |
Completeness [%] | 98.0 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 200 MM SODIUM MALONATE, 20 % (V/V) PEG 3350, pH 7 |