4ZZL
MexR R21W derepressor mutant causing multidrug resistance in P. aeruginosa by mexAB-oprM efflux pump expression
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-07 |
Detector | DECTRIS PIXEL |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 84.880, 84.880, 114.187 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.090 - 2.190 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.20620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lnw |
RMSD bond length | 0.010 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.090 | 2.310 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.090 | 0.900 |
Number of reflections | 24956 | |
<I/σ(I)> | 24.9 | 4.7 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 21.2 | 21.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |