4YHH
Crystal structure of the 30S ribosomal subunit from Thermus thermophilus in complex with tigecycline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-02-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 409.590, 409.590, 171.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.716 - 3.417 |
R-factor | 0.2312 |
Rwork | 0.228 |
R-free | 0.28740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zm6 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.308 |
Data reduction software | XDS (July 4, 2012) |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 6.995 | |
Number of reflections | 192315 | |
<I/σ(I)> | 4.45 | 0.3 |
Completeness [%] | 96.7 | 84.3 |
Redundancy | 4.5 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | HEPES-KOH, MgCl2, NH4Cl, beta-mercaptoethanol, MPD, spermidine |