4YD2

Nicked complex of human DNA Polymerase Mu with 2-nt gapped DNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2012-09-16
Detector	RIGAKU SATURN 92
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	60.083, 68.700, 114.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.613 - 2.471
R-factor	0.214
Rwork	0.212
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yd1
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge		0.500
Number of reflections	17102
<I/σ(I)>	16.15	2.82
Completeness [%]	99.5	98.6
Redundancy	5.1	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	0.1M Tris, 20% PEG8K, 0.2M MgCl2