4WNI
Crystal structure of the T229K mutant of human GAPDH at 2.3 angstroems resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.127 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.363, 133.095, 147.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.500 - 2.300 |
R-factor | 0.169 |
Rwork | 0.169 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u8f |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.500 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.235 | |
Number of reflections | 63669 | |
<I/σ(I)> | 10.7 | 12.1 |
Completeness [%] | 88.5 | 55.4 |
Redundancy | 4.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% (w/v) PEG3350, 0.02 M ZnCl2 |