4V9N
Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Collection date | 2012-08-02 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 212.070, 454.400, 618.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.672 - 3.400 |
R-factor | 0.235 |
Rwork | 0.234 |
R-free | 0.26810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3F1G AND 3F1H |
RMSD bond length | 0.004 |
RMSD bond angle | 0.621 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.400 |
Number of reflections | 807927 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7.0 | ||
1 | 7 | pH 7.0 | ||
1 | 7 | pH 7.0 | ||
1 | 7 | pH 7.0 |