4V06
Crystal structure of human tryptophan hydroxylase 2 (TPH2), catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 95.960, 100.199, 89.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.504 - 2.630 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pah |
RMSD bond length | 0.002 |
RMSD bond angle | 0.531 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.500 | 2.750 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.120 | 1.320 |
Number of reflections | 26141 | |
<I/σ(I)> | 11.9 | 2.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.6 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.20M SODIUM ACETATE; 0.1M BIS-TRIS- PROPANE PH 6.5; 20.0% PEG 3350; 10.0% ETHYLENE GLYCOL |