4UU9
Crystal structure of the human c5a in complex with MEDI7814 a neutralising antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-13 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.854, 117.472, 126.457 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.070 - 2.120 |
R-factor | 0.17595 |
Rwork | 0.175 |
R-free | 0.20163 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.174 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.000 | 2.240 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.130 | 0.590 |
Number of reflections | 48925 | |
<I/σ(I)> | 19 | 8.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.2 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG10K, 8% ETHYLENE GLYCOL, 100MM HEPES PH 7.5 |