4UOY
Crystal structure of YgjG in complex with Pyridoxal-5'-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-03 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 120.945, 129.319, 131.102 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.746 - 2.305 |
R-factor | 0.1818 |
Rwork | 0.181 |
R-free | 0.23340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vef |
RMSD bond length | 0.009 |
RMSD bond angle | 1.136 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 91029 | |
<I/σ(I)> | 13.4 | 3 |
Completeness [%] | 98.3 | 96.9 |
Redundancy | 6.1 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES PH 7.5 15% PEG 3350 0.2 M SODIUM FORMATE 0.1 MM N-DODECYL-N,N-DIMETHYLGLYCINE |