4U16
M3-mT4L receptor bound to NMS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-03-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.391, 187.180, 53.548 |
Unit cell angles | 90.00, 99.78, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 3.830 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.219 | 0.884 |
Number of reflections | 14787 | |
<I/σ(I)> | 8.9 | 2.2 |
Completeness [%] | 93.7 | 89.1 |
Redundancy | 2.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | The best crystallization condition was 100 mM Tris pH 7.5, 44% PEG 300 and 400 mM ammonium tartrate. |