4R2P
Wilms Tumor Protein (WT1) zinc fingers in complex with hydroxymethylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.852, 78.591, 35.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.242 - 1.788 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2e |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.472 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.242 | 1.850 |
| High resolution limit [Å] | 1.788 | 1.790 |
| Rmerge | 0.067 | 0.428 |
| Number of reflections | 18227 | |
| <I/σ(I)> | 27.3 | 3.8 |
| Completeness [%] | 99.0 | 92.9 |
| Redundancy | 8.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 20% (w/v) PEG 10000, 0.1 M Hepes-NaOH pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
