4PO7
Structure of the Sortilin:neurotensin complex at excess neurotensin concentration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-5 |
Synchrotron site | MAX II |
Beamline | I911-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.90736 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.010, 78.650, 110.980 |
Unit cell angles | 90.00, 126.62, 90.00 |
Refinement procedure
Resolution | 20.968 - 2.660 |
R-factor | 0.1684 |
Rwork | 0.166 |
R-free | 0.21190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.756 |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.970 | 2.730 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.054 | 0.744 |
Number of reflections | 32040 | |
<I/σ(I)> | 16.79 | 2.1 |
Completeness [%] | 98.6 | 99 |
Redundancy | 3.7 | 3.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 292 | 20% PEG 6k, 300 mM sodium malonate, 3% glycerol and 100 mM TrisHCl, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 292K |