4P59
HER3 extracellular domain in complex with Fab fragment of MOR09825
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 124.226, 140.939, 180.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.030 - 3.400 |
| R-factor | 0.1874 |
| Rwork | 0.184 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.240 |
| Refinement software | BUSTER-TNT (BUSTER 2.11.4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 111.030 | 111.027 | 3.580 |
| High resolution limit [Å] | 3.400 | 10.750 | 3.400 |
| Rmerge | 0.024 | 0.461 | |
| Rmeas | 0.139 | ||
| Rpim | 0.053 | 0.011 | 0.208 |
| Total number of observations | 148197 | 4807 | 21588 |
| Number of reflections | 22112 | ||
| <I/σ(I)> | 14.6 | 54.1 | 3.8 |
| Completeness [%] | 99.9 | 98.9 | 99.9 |
| Redundancy | 6.7 | 6.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM bis-tris pH 6.5, 16% (w/v) PEG 10000 |
