4NUJ
Crystal structure of HIV-1 broadly neutralizing antibody PGT152
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.990, 66.300, 84.878 |
| Unit cell angles | 90.00, 134.12, 90.00 |
Refinement procedure
| Resolution | 32.859 - 1.827 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nug |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.173 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 42208 |
| <I/σ(I)> | 28.9 |
| Completeness [%] | 99.9 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 293.15 | 40% PEG 600, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, temperature 293.15K |
