4M9N
DNA Polymerase Beta E295K Soaked with dATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 104 |
Detector technology | IMAGE PLATE |
Collection date | 2013-01-16 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.523, 79.215, 54.685 |
Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
Resolution | 13.923 - 2.275 |
R-factor | 0.1951 |
Rwork | 0.192 |
R-free | 0.22670 |
Structure solution method | Isomorphous replacement |
RMSD bond length | 0.002 |
RMSD bond angle | 0.598 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 14.000 | 14.000 | 2.360 |
High resolution limit [Å] | 2.275 | 4.850 | 2.275 |
Rmerge | 0.053 | 0.026 | 0.245 |
<I/σ(I)> | 17.8 | ||
Completeness [%] | 97.5 | 99.9 | 81.9 |
Redundancy | 3.3 | 3.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 14% PEG 3350, 300 mM sodium acetate pH 9, 50 mM HEPES pH 7.5, vapor diffusion, hanging drop, temperature 291K |