4LY6
Nucleotide-induced asymmetry within ATPase activator ring drives s54-RNAP interaction and ATP hydrolysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 119.339, 130.037, 206.368 |
Unit cell angles | 90.00, 89.73, 89.90 |
Refinement procedure
Resolution | 37.855 - 3.600 |
R-factor | 0.2603 |
Rwork | 0.258 |
R-free | 0.30770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.087 |
Data reduction software | d*TREK (9.7 W8RSSI) |
Data scaling software | d*TREK (9.7 W8RSSI) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.860 | 37.860 | 3.730 |
High resolution limit [Å] | 3.600 | 7.740 | 3.600 |
Rmerge | 0.084 | 0.038 | 0.363 |
Number of reflections | 109790 | ||
<I/σ(I)> | 5 | 13.4 | 1.2 |
Completeness [%] | 76.4 | 84.8 | 44.3 |
Redundancy | 1.66 | 1.66 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 277 | 20% ethylene glycol, pH 7.9, vapor diffusion, hanging drop, temperature 277K |