4K4T
Poliovirus polymerase elongation complex (r4_form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | NOIR-1 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 59.401, 59.248, 97.455 |
Unit cell angles | 78.11, 78.09, 78.85 |
Refinement procedure
Resolution | 46.959 - 2.750 |
R-factor | 0.2047 |
Rwork | 0.202 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.216 |
Data reduction software | d*TREK (9.9.9.1L) |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.960 | 46.960 | 2.850 |
High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
Rmerge | 0.069 | 0.064 | 0.468 |
Total number of observations | 6084 | 6050 | |
Number of reflections | 31399 | ||
<I/σ(I)> | 6.8 | 14.9 | 1.8 |
Completeness [%] | 96.1 | 96.1 | 95.6 |
Redundancy | 1.91 | 1.92 | 1.91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.16 M MgCl2, 0.08 M Tris-HCl, 24% (w/v) PEG 4000, 20% (v/v) glycerol and directly frozen for data collection , VAPOR DIFFUSION, SITTING DROP, temperature 289K |