4JKB
Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.355, 78.159, 93.766 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.040 - 1.300 |
R-factor | 0.1207 |
Rwork | 0.119 |
R-free | 0.14530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3enb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.442 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.6.0117) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.350 |
High resolution limit [Å] | 1.300 | 2.800 | 1.300 |
Rmerge | 0.041 | 0.027 | 0.568 |
Number of reflections | 135702 | ||
<I/σ(I)> | 13.3 | ||
Completeness [%] | 99.4 | 94.5 | 100 |
Redundancy | 5.5 | 5.2 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K |