4JA1
Structure of MMP3 complexed with a platinum-based inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.119, 77.615, 106.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.220 - 1.960 |
| R-factor | 0.19957 |
| Rwork | 0.195 |
| R-free | 0.24361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sln |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | ILMILIONE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.220 | 3.340 | 2.050 |
| High resolution limit [Å] | 1.960 | 2.890 | 1.960 |
| Number of reflections | 21159 | ||
| Completeness [%] | 99.9 | 100 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Protein 7-15mg/ml, 25-30% (w/v) PEG 8000, 10 mM CaCl2, 100 mM TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
