4IWP
Crystal structure and mechanism of activation of TBK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.28 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 135.410, 135.410, 86.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.510 - 3.065 |
| R-factor | 0.2826 |
| Rwork | 0.280 |
| R-free | 0.33460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.509 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 17416 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 5% PEG6000, pH 7.5, vapor diffusion, hanging drop, temperature 294K |
