4IQX
Mutant P44S P169S M296I of Foot-and-mouth disease Virus RNA-dependent RNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.977 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 93.836, 93.836, 99.867 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.360 - 2.500 |
R-factor | 0.22341 |
Rwork | 0.222 |
R-free | 0.25389 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.855 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.078 | 0.300 |
Number of reflections | 18047 | |
<I/σ(I)> | 14.1 | 5.7 |
Completeness [%] | 99.3 | 100 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 26% PEG 4000, 0.2M magnesium acetate, 6% butyrolactone, 0.1M MES , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |