4HAB
Crystal structure of Plk1 Polo-box domain in complex with PL-49
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 95.043, 95.043, 240.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.650 |
R-factor | 0.2603 |
Rwork | 0.260 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 2.026 |
Data scaling software | SCALA (3.3.20) |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 88.277 | 88.277 | 2.790 |
High resolution limit [Å] | 2.650 | 8.380 | 2.650 |
Rmerge | 0.031 | 0.348 | |
Number of reflections | 32192 | ||
<I/σ(I)> | 14.4 | 19.2 | 2 |
Completeness [%] | 98.6 | 90.6 | 99.8 |
Redundancy | 4 | 3.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 10 | 293 | 0.1M CAPS pH 8, 0.2M LiSO4, 0.8M K2HPO4 NaH2PO4, vapor diffusion, temperature 293K |