4H75
Crystal structure of human Spindlin1 in complex with a histone H3K4(me3) peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9789 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 122.429, 42.685, 50.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.866 - 2.098 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ns2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.064 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.098 | 2.098 |
Rmerge | 0.077 | 0.387 |
Number of reflections | 16119 | |
<I/σ(I)> | 24.3 | 6.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 289 | 0.1 M CHES, pH 9.5, 2.1 M ammonium sulfate, 0.2 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |