4H5J
Crystal Structure of the Guanine Nucleotide Exchange Factor Sec12 (P64 form)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792, 0.9794, 0.9640 |
Spacegroup name | P 64 |
Unit cell lengths | 189.473, 189.473, 53.424 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.368 - 2.601 |
R-factor | 0.1807 |
Rwork | 0.179 |
R-free | 0.21640 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.189 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (dev_1144) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.120 | 0.072 | 0.446 |
Number of reflections | 34159 | ||
<I/σ(I)> | 7.6 | ||
Completeness [%] | 99.9 | 99.7 | 99.8 |
Redundancy | 8.3 | 8.9 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.3 | 298 | 91 mM Tris, pH 8.0, 181 mM potassium citrate, pH 8.3, 22% w/v PEG3350, VAPOR DIFFUSION, temperature 298K |