4GUD
Crystal Structure of Amidotransferase HisH from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.422, 118.234, 42.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.143 - 1.911 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.20800 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.889 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.7.3_920)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 33885 | |
<I/σ(I)> | 8.9 | 3.9 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 5.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Calcium Chloride, 0.1M TRIS HCl pH 8.5, 25% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |