4GHA
Crystal structure of Marburg virus VP35 RNA binding domain bound to 12-bp dsRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-05-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.593, 99.191, 81.801 |
Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
Resolution | 81.800 - 2.500 |
R-factor | 0.21073 |
Rwork | 0.209 |
R-free | 0.24987 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gh9 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.448 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.800 | 2.566 |
High resolution limit [Å] | 2.500 | 2.501 |
Rmerge | 0.131 | 0.362 |
Number of reflections | 20261 | |
<I/σ(I)> | 8.5 | 2.2 |
Completeness [%] | 100 | |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Bis-Tris, 2% v/v Tacsimate, 18% PEG 3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |