4FTV
The complex between the high affinity version of A6 TCR (A6c134) and human Class I MHC HLA-A2 with the bound TAX nonameric peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-12 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.98494 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 224.230, 48.954, 94.398 |
Unit cell angles | 90.00, 89.62, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.740 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.26040 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.378 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.095 | 0.557 |
Number of reflections | 26730 | |
<I/σ(I)> | 14.9 | |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG3350 24%, MES 0.025M, NaCl 0.1M, pH 6.5, vapor diffusion, sitting drop, temperature 277K |