4FBW

Crystal structure of an unfused Mre11-Nbs1 complex with two manganese ions per active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	59.122, 79.955, 220.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.537 - 2.200
R-factor	0.2335
Rwork	0.233
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.766
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.537
High resolution limit [Å]	2.200
Number of reflections	53880
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			200 mM ammonium citrate pH 5.5 14 % PEG 3350 50 mM MnCl2, VAPOR DIFFUSION, HANGING DROP