4F63
Crystal structure of Human Fibroblast Growth Factor Receptor 1 Kinase domain in complex with compound 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-12-22 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54179 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.306, 57.477, 65.452 |
Unit cell angles | 90.00, 107.42, 90.00 |
Refinement procedure
Resolution | 17.330 - 2.550 |
R-factor | 0.23223 |
Rwork | 0.229 |
R-free | 0.28741 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fgk |
RMSD bond length | 0.013 |
RMSD bond angle | 1.421 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.330 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.093 | 0.710 |
Number of reflections | 23652 | |
<I/σ(I)> | 8.76 | 1.82 |
Completeness [%] | 97.0 | 97.84 |
Redundancy | 2.63 | 2.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 16-20% PEG8000, 100 mM PCTP, 100-300 mM ammonium sulfate, 25% ethylene glycol, pH 6.25-7.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |