4F5I
Substrate Specificity Conversion of E. coli Pyridoxal-5'-Phosphate Dependent Aspartate Aminotransferase to Tyrosine Aminotransferase: Chimera P4.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2010-07-14 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.959, 103.065, 138.834 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.217 - 2.200 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.039 |
Data reduction software | PROTEUM PLUS (PLUS) |
Data scaling software | PROTEUM PLUS (PLUS) |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.290 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.056 | 0.311 |
Number of reflections | 44527 | |
<I/σ(I)> | 18.07 | 3.34 |
Completeness [%] | 99.9 | 99.2 |
Redundancy | 11.14 | 6.41 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 8% PEG 4000, 0.1 M Sodium Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |