4E6I
Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-06 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 41 |
Unit cell lengths | 68.337, 68.337, 63.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.520 - 2.000 |
R-factor | 0.2166 |
Rwork | 0.211 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rdf |
RMSD bond length | 0.018 |
RMSD bond angle | 1.978 |
Data reduction software | XPREP (2008/2 for Windows) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.610 | 31.610 | 2.040 |
High resolution limit [Å] | 2.000 | 5.500 | 2.000 |
Rmerge | 0.032 | 0.010 | 0.503 |
Number of reflections | 19825 | 923 | 1048 |
<I/σ(I)> | 23.95 | ||
Completeness [%] | 100.0 | 99.1 | 100 |
Redundancy | 29.77 | 42.5 | 16.57 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |