4CRV
Complex of human CNOT9 and CNOT1 including two tryptophans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2013-08-19 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.070, 66.810, 71.990 |
Unit cell angles | 90.00, 100.22, 90.00 |
Refinement procedure
Resolution | 61.138 - 2.050 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cru |
RMSD bond length | 0.006 |
RMSD bond angle | 0.883 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.100 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 45149 | |
<I/σ(I)> | 14.9 | 1.8 |
Completeness [%] | 99.5 | 99 |
Redundancy | 6.8 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.1M MES (PH=6.0), 11% PEG6000, 0.05M MG-CHLORIDE, 0.04M L-TRYPTOPHAN |