4B9T
Structure of the high fidelity DNA polymerase I with an oxidative formamidopyrimidine-dG DNA lesion -dC basepair in the post-insertion site.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-28 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.381, 93.775, 105.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.650 |
R-factor | 0.22395 |
Rwork | 0.221 |
R-free | 0.27302 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u45 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.181 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.300 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.100 | 0.470 |
Number of reflections | 24741 | |
<I/σ(I)> | 17.6 | 4 |
Completeness [%] | 97.1 | 97.1 |
Redundancy | 5.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |