4B3S
Crystal structure of the 30S ribosome in complex with compound 37
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-06 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 401.150, 401.150, 173.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.150 |
R-factor | 0.2104 |
Rwork | 0.210 |
R-free | 0.24730 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1j5e |
RMSD bond length | 0.007 |
RMSD bond angle | 1.239 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.250 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.170 | 0.960 |
Number of reflections | 240081 | |
<I/σ(I)> | 8.2 | 2.1 |
Completeness [%] | 99.2 | 99.5 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 4% MPD, 75MM NH4CL, 200MM KCL, 15MM MAGNESIUM CHLORIDE, MES-KOH PH 6.5,VAPOR DIFFUSION, HANGING DROP AT 277K |