3VM8
Crystal structure of the human APOBEC3C having HIV-1 Vif-binding interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-05-27 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 |
Unit cell lengths | 104.419, 104.419, 74.502 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 90.430 - 3.000 |
R-factor | 0.2315 |
Rwork | 0.228 |
R-free | 0.30230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ir2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.992 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 1000.000 | 1000.000 | 3.050 |
High resolution limit [Å] | 3.000 | 8.140 | 3.000 |
Rmerge | 0.108 | 0.050 | 0.522 |
Number of reflections | 9105 | ||
<I/σ(I)> | 13.9 | ||
Completeness [%] | 97.0 | 96.6 | 100 |
Redundancy | 10.2 | 10.1 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Bicine, PEG 6000, L-Arginine HCl, pH 9.0, vapor diffusion, hanging drop, temperature 293K |