3U85
Crystal structure of human menin in complex with MLL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 141.205, 141.205, 93.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.282 - 3.000 |
| R-factor | 0.224 |
| Rwork | 0.220 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.809 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.088 | 0.060 | 0.334 |
| Number of reflections | 18712 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 94.6 | 98.2 | 75.5 |
| Redundancy | 23.7 | 22.3 | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2.3 M NaCl, pH 7.0, vapor diffusion, sitting drop, temperature 277K |
