3TF5
Structure of UDP-glucose dehydrogenase V132 deletion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2010-12-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.030, 116.930, 96.340 |
| Unit cell angles | 90.00, 115.93, 90.00 |
Refinement procedure
| Resolution | 37.715 - 2.300 |
| R-factor | 0.2022 |
| Rwork | 0.201 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_833)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.430 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.104 | |
| Number of reflections | 77497 | |
| <I/σ(I)> | 18.13 | 2.5 |
| Completeness [%] | 96.0 | 91 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 295 | 0.1 mM piperine, 12% PEG3350, 2% DMSO, 125 mM magnesium acetate, 0.1 M sodium glycine, pH 9.4, yielded triangular plates within one week, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
