3PUZ
Crystal Structure of a pre-translocation state MBP-Maltose transporter complex bound to AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03326 |
Spacegroup name | P 1 |
Unit cell lengths | 80.021, 93.360, 119.761 |
Unit cell angles | 90.05, 102.57, 105.01 |
Refinement procedure
Resolution | 19.950 - 2.900 |
R-factor | 0.23928 |
Rwork | 0.237 |
R-free | 0.27878 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.894 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.043 | |
Number of reflections | 53826 | |
<I/σ(I)> | 1.7 | |
Completeness [%] | 71.7 | 23.6 |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 32% PEG 400, 0.1 M HEPES, 40 mM magnesium chloride, 50 mM sodium chloride , VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.5 |