3PNA
Crystal Structure of cAMP bound (91-244)RIa Subunit of cAMP-dependent Protein Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2008-12-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 62.642, 62.642, 158.197 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.259 - 1.503 |
| R-factor | 0.1582 |
| Rwork | 0.157 |
| R-free | 0.18220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.137 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.300 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.057 | 0.490 |
| Number of reflections | 56705 | |
| <I/σ(I)> | 16.8 | 4.9 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1 M sodium acetate trihydrate, 2.0 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
