3OXU

Complement components factor H CCP19-20 and C3d in complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	74.675, 82.990, 85.605
Unit cell angles	112.75, 110.14, 99.96

Refinement procedure

Resolution	40.482 - 2.100
R-factor	0.1724
Rwork	0.171
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2G7I 1c3d
RMSD bond length	0.007
RMSD bond angle	1.018
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.053	0.465
Number of reflections	93199
<I/σ(I)>	14.9	5.1
Completeness [%]	96.4	96.4
Redundancy	4.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	290	4% w/v PEG 8000, 0.1 M Tris HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K