3OS5
SET7/9-Dnmt1 K142me1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.359, 38.535, 66.541 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.680 - 1.690 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23800 |
| Structure solution method | MIR |
| Starting model (for MR) | 3cbm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.680 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.068 | 0.604 |
| Number of reflections | 30160 | |
| <I/σ(I)> | 21 | 2.2 |
| Completeness [%] | 99.5 | 95.4 |
| Redundancy | 6.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 289 | 40% PEG3350, 100 mM Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
