3L4K
Topoisomerase II-DNA cleavage complex, metal-bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.2823 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 86.064, 91.851, 115.939 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.023 - 2.980 |
R-factor | 0.2408 |
Rwork | 0.239 |
R-free | 0.27330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.250 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.070 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.102 | 0.538 |
Number of reflections | 18071 | |
<I/σ(I)> | 10.6 | 2.1 |
Completeness [%] | 91.4 | 94 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 20% 1,4-BUTANEDIOL, 0.1M SODIUM ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |