Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-05 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.810, 71.700, 131.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.868 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uzk |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.924 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.938 | 1.960 |
| High resolution limit [Å] | 1.868 | 1.868 |
| Rmerge | 0.044 | 0.251 |
| Total number of observations | 12693 | |
| Number of reflections | 16135 | |
| <I/σ(I)> | 12.2 | 3 |
| Completeness [%] | 96.6 | 81.6 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 292 | HEPES, MgCl2, PEG MME, pH 7.3, vapor diffusion, hanging drop, temperature 292K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | PEG MME | ||
| 4 | 1 | 2 | HEPES | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | PEG MME |
