3K7A
Crystal Structure of an RNA polymerase II-TFIIB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-08-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 204.150, 216.210, 420.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.800 |
| R-factor | 0.268 |
| Rwork | 0.263 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1R5U WITHOUT CHAIN M |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.363 |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 210.490 | 149.070 | 4.010 |
| High resolution limit [Å] | 3.800 | 12.020 | 3.800 |
| Rmerge | 0.093 | 0.034 | 0.545 |
| Rmeas | 0.040 | 0.750 | |
| Rpim | 0.020 | 0.370 | |
| Total number of observations | 10790 | 50783 | |
| Number of reflections | 89990 | ||
| <I/σ(I)> | 9.6 | 32.7 | 2 |
| Completeness [%] | 98.6 | 96.1 | 99.3 |
| Redundancy | 3.9 | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 295 | 1.2 M sodium/potassium phosphate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
